Fig. 2

Pre-treatment with prodigiosin impairs Golgi apparatus reassembly after BFA treatment. HeLa wt cells were seeded on cover slips. On the next day, cells were treated with DMSO, 10 nM prodigiosin or 100 nM prodigiosin for 24 h. Cells were washed once and treated with 5 μg/mL brefeldin A (BFA) or DMSO (ct) for 2 h. BFA was washed out with DPBS 4 times and cells were incubated in fresh growth medium to wash out (W/O) BFA for 0/15/30/45/60/120 min. After treatment, cover slips were prepared for microscopy. A Representative sections are depicted. Scale bar: 10 µm. B-E The relative number per cell and mean area of TGN46 and B4GALT1 positive structures of 15 representative images from three biological replicates for each treatment were quantified using ImageJ 1.53c