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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: A missense mutant of ocrl1 promotes apoptosis of tubular epithelial cells and disrupts endocytosis and the cell cycle of podocytes in Dent-2 Disease

Fig. 5

Identification of proteins interacting with OCRL1. A, Kyoto Encyclopedia of Genes and Genomes analysis using RNA sequencing data from podocyte cells. B and C, Western blot analysis demonstrates the effect of ocrl1 knockdown on the protein levels of the cell cycle pathway in podocytes (n = 3). Immunoprecipitation and protein pull-down analysis revealed the interaction between ocrl1, CCND1, E2F1, and CDKN2D. D, Co-immunoprecipitation experiments were performed using control antibody (IgG) or antibodies to OCRL1, CCND1, E2F1, and CDKN2D to demonstrate the interaction between the endogenous proteins. OCRL1 and E2F1 showed a relatively close interaction

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Cell Communication and Signaling

ISSN: 1478-811X