Fig. 3From: A missense mutant of ocrl1 promotes apoptosis of tubular epithelial cells and disrupts endocytosis and the cell cycle of podocytes in Dent-2 DiseaseKnockdown of ocrl1 and overexpression of the ocrl1 mutant induced phosphatidylserine eversion and crystal adhesion in HK-2 cells. A, HK-2 cells were transfected as indicated. Flow cytometry analysis was performed to measure phosphatidylserine eversion. Data were expressed as the mean ± SEM. *P < 0.05 vs. control; n = 3. B, Scanning electron microscopy was used to examine the adhesion of HK-2 cells to calcium oxalate monohydrate. si-ocrl1, siRNA targeting ocrl1; H318, adenoviral vectors overexpressing ocrl1 mutantBack to article page