Table 1 Primers used for qPCR in this study
Target gene | Forward primer sequence (5’ – 3’) | Reverse primer sequence (5’ – 3’) | Annealing temp. (°C) |
|---|---|---|---|
mTOR | ACCGGCACACATTTGAAGAAG | CACCACCAAGGATAAGGTAG | 52.4 |
ULK1 | AGGATGGGGACTTGGTTGC | CGATGTTTTCGTGCTTTAGTTCC | 52.4 |
PI3K | CCTGGACATCAACGTGCAG | TGTCTCTTGGTATAGCCCAGAAA | 53.2 |
BECN1 | AGTTGAGAAAGGCGAGACAC | CACCACCAAGGATAAGGTAG | 54.4 |
BECN2 | GTCGCTACCGTCGTGACTTC | CAGACATGCACCTACCCAGC | 55.9 |
ATG16L | CAGAGCAGCTACTAAGCGACT | AAAAGGGGAGATTCGGACAGA | 52.4 |
ATG13 | CAGAACTGCTGGTGAGGACACT | AGCAGGCTGATAGGAAAGGCGA | 56,7 |
ATG5 | AGCAACTCTGGATGGGATTG | CACTGCAGAGGTGTTTCCAA | 55.3 |
LC3 | CGAGAGCAGCATCCTACCAA | TTCTTCCGCGAATGTCGAGT | 51.8 |
ACTB | CATCGGCAATGAGCGGTTCC | CCGTGTTGGCGTAGAGGTCC | 68.1 |