Fig. 1

ALS- and LPS-induced endoplasmatic reticulum stress response in the mouse testis. (A) Schematic presentation of the ER-mediated stress response pathway in the testis after administration of ALS; expression of proteins involved in ATF6, PERK and IRE1 pathways examined by Western blot method (B); i.e.: ATF6 (C), p-PERK (D), p-IRE1α (E), p-ASK1 (F), p-TRAF2 (G), p-p38MAPKα (H), p-eIF2α (I), p-ATF4 (J), p-CHOP (K), GADD34 (L); Bars indicate mean \(\pm\) SD, n = 7 per each group, (*) indicate comparison between control and analyzed groups after MTEP, NS-398, IMI, MTEP + NS-398, IMI + NS-398 with and without LPS; ***/^^^ p < 0.001, **/^^ p < 0.01, */^; (^) indicate comparison after administration the same substances between the groups without and with LPS; no indication = no statistical significance (one-way ANOVA with Dunnett’s a post hoc test). The results were normalized to β-actin