Fig. 4
From: LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell migration
LPA reduces CXCR4-mediated Gαi activation. A Schematic diagram of measuring Gαi recruitment to GPCR by BRET. B HEK293A cells were cotransfected with Gαi1-Rluc8, Gβ1, Gγ1, and CXCR4-mCitrine alone or together with Flag-LPA1. BRET between CXCR4-mCitrine and Gαi1-Rluc8 was measured after stimulation with CXCL12 (10 nM) or LPA (1 μM). C HEK293A cells were cotransfected with Gαi1-Rluc8, Gβ1, Gγ1, and Flag-LPA1-mCitrine alone or together with Myc-CXCR4. BRET between Flag-LPA1-mCitrine and Gαi1-Rluc8 was measured after stimulation with CXCL12 (10 nM) or LPA (1 μM). Data represent the mean ± SEM of n = 3 independent experiments performed in triplicate. Statistical significance was tested using unpaired two-tailed Student’s t test. **P < 0.01; ***P < 0.001