Fig. 5

miR-24-3p inhibited the JAK/STAT and NF-κB signaling pathways: A DENN/MADD knockdown by lenti-siRNA-DENN/MADD transfection significantly increased HD T-cell apoptosis levels compared to the control (n = 5 **p < 0.003). Lenti-siRNA was included as a control. B R-EXO-induced apoptosis is rescued by DENN/MADD overexpression in HD CD3 T cells. C Western blot analysis of the NF-κB and JAK/STAT signaling pathways. The overexpression of miR-24-3p by T-cell transfection by miR-24-3p mimic decreased the expression levels of NF-κB and TNFR1, while its inhibition increased their expression. miR-24-3p decreases the expression of p-ERK protein. D The overexpression of miR-24-3p after transfection with the miR-24-3p mimic decreased the levels of p-JAK1, p-STAT1, and p-STAT3, which were recovered by the inhibition of miR-24-3p by the miR-24-3p inhibitor mimic. The β-actin gene was included as a control. E The levels of p-NFKB and p-ERK were lower in lenti-siRNA-DENN/MADD-transduced T- cells than in lenti-siRNA control cells. F The level of the p-ERK protein was lower in lenti-siRNA-DENN/MADD-transduced T cells than in lenti-siRNA control-transfected T cells. For (C, D, E, and F), the figure shows representative Western blot data from three independent experiments. A Bar graphs represent the mean ± SEM *p < 0.05, **p < 0.01, ***p < 0001