Fig. 7

MAO-B plays a vital role in inducing NETs formation and promoting neuroinflammation. A. Representative blots and quantification of MAO-B in the spinal cords of sham and CFA mice (n = 5, F_{4, 20} = 6.445, P = 0.0017). B. ELISA detecting 5-HIAA in the spinal dorsal horns (n = 5, F_{4, 20} = 8.389, P = 0.0004). C. Representative blots and quantification of GPR35 in the spinal cords of sham and CFA groups (n = 5, F_{4, 20} = 3.227, P = 0.0338). D–E. Double immunofluorescence staining for MAO-B (red) and GFAP (green) or NeuN (green) in spinal cords (n = 3, scale bars: 50 or 100 μm). F and G. ROS production in the spinal dorsal horns assessed by flow cytometry using DCFH-DA (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, compared with the sham mice