Fig. 10

Specific knockdown of neuron and astrocyte MAO-B alleviates inflammatory pain. A. Schematic paradigm of MAO-B knockdown in spinal cord astrocytes of CFA mice. B. Fluorescence images of viral particles in the spinal cord of CFA mice treated with AAV-GFAP-shMAO-B virus (n = 3, scale bars: 50 μm). C. Representative blots and quantification of MAO-B in the spinal cords of mice (n = 3). D. Paw withdrawal threshold of mice in each group (n = 6, group: F_{3, 15} = 69.43, P < 0.0001; time: F_{4, 20} = 116.6, P < 0.0001; interaction: F_{12, 60} = 9.147, P < 0.0001). E. Paw withdrawal latency of mice in each group (n = 6, group: F_{3, 15} = 107.5, P < 0.0001; time: F_{4, 20} = 304, P < 0.0001; interaction: F_{12, 60} = 7.103, P < 0.0001). F. Schematic paradigm of MAO-B knockdown in spinal cord neurons of CFA mice. G. Fluorescence images of viral particles in the spinal cord of CFA mice treated with AAV-hSyn-shMAO-B virus (n = 3, scale bars: 50 μm). H. Representative blots and quantification of MAO-B in the spinal cords of mice (n = 3). I. Paw withdrawal threshold of mice in each group (n = 6, group: F_{3, 15} = 57.55, P < 0.0001; time: F_{4, 20} = 117.5, P < 0.0001; interaction: F_{12, 60} = 11.5, P < 0.0001). J. Paw withdrawal latency of mice in each group (n = 6, group: F_{3, 15} = 138.3, P < 0.0001; time: F_{4, 20} = 227.5, P < 0.0001; interaction: F_{12, 60} = 8.401, P < 0.0001). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, compared with the AAV-NC or vehicle group mice. #P < 0.05, compared with the selegiline group mice