Fig. 6

TLR7 inhibition alleviates renal damage and inflammation in the folic acid-treated mouse model. A Study design of the administration of M5049 (0.3 mg/kg/day) to the folic acid-induced kidney fibrosis model. B Serum BUN levels of folic acid-treated mice with or without M5049 administration. #p < 0.05 versus control mice. *p < 0.05 versus folic acid-treated mice. C Relative mRNA levels of kidney damage-related genes (Havcr1 and Lcn2) in the kidney of folic acid-treated mice with or without M5049 administration. #p < 0.05 versus control mice. *p < 0.05 versus folic acid-treated mice. D Representative H&E staining images of folic acid-treated kidneys with or without M5049 administration. Scale bar = 50 μm. E Relative mRNA levels of pro-inflammatory cytokines (Tnfa, Il6, Ccl2, and Cxcl1) and inflammatory cell marker genes (Cd68 and Emr1) in the kidney of folic acid-treated mice with or without M5049 administration. #p < 0.05 versus control mice. *p < 0.05 versus folic acid-treated mice. F Protein levels of p65, p-p65, CD3, and CD68 in the kidneys were determined via western blotting. α-tubulin was used as a loading control. G Representative ISH images of Ccl2 (red) in the kidneys. Scale bar = 50 μm. H Representative IHC images of p-p65 in the kidneys. Scale bar = 50 μm