Fig. 3

TLR7-deficient kidneys are protected from inflammation. A Relative mRNA levels of pro-inflammatory cytokines (Tnfa, Il1b, Il6, Ccl2, and Ccl3) in the kidneys of WT and TLR7 KO mice fed a chow diet or adenine diet for 3 weeks. #p < 0.05 versus chow diet-fed WT mice. *p < 0.05 versus adenine diet-fed WT mice. B Relative mRNA levels of inflammatory cell marker genes (Cd68 and Emr1) in the kidneys of WT and TLR7 KO mice fed a chow diet or adenine diet for 3 weeks. #p < 0.05 versus chow diet-fed WT mice. *p < 0.05 versus adenine diet-fed WT mice. C Protein levels of p65 and phosphorylated-p65 in the kidneys were determined. α-tubulin was used as a loading control. D  Representative IHC images of phosphotylated-p65 expression in the kidneys. Scale bar = 50 μm. E Protein levels of CD68 and CD3 were determined in the kidneys. α-tubulin was used as a loading control. F Representative ISH images of Emr1 (Red) gene in the kidneys of WT and TLR7 KO mice fed chow diet or adenine diet. Scale bar = 50 μm