Fig. 1

Changes in the TLR family gene and protein levels in adenine diet-induced renal fibrosis model. A Relative mRNA levels of the TLR family genes (Tlr1, Tlr2, Tlr3, Tlr4, Tlr5, Tlr7, Tlr8, and Tlr9) in the kidneys of mice fed normal chow diet or adenine diet for 3 weeks. *p < 0.05 versus normal chow diet-fed mice. B TLR2, TLR4, TLR5, TLR7, and TLR8 protein levels in kidneys were detected using western blotting. α-tubulin was used as a loading control. C Representative immunohistochemical (IHC) images of TLR7 expression in kidneys of normal chow- or adenine diet-fed mice. Scale bar = 50 μm. D Representative in situ hybridization (ISH) images of the Tlr7 (Green) and Emr1 (Red) genes in the kidney of mice fed chow diet or adenine diet. Scale bar = 50 μm. E Representative ISH images of the Tlr7 (Green) and Col1a1 (Red) genes in the kidney of mice fed chow diet or adenine diet. Scale bar = 50 μm. F Representative ISH images of the Col1a1 (Green) and Emr1 (Red) genes in the kidney of mice fed chow diet or adenine diet. Scale bar = 50 μm