Fig. 4

Golgi disruption reduces bacterial adherence but improves replication. A BEAS-2B cells were stimulated with Golgicide A, 4 µM or DMSO for 24 h. Afterwards, cells were infected with Spn D39, MOI 20 for 2 h. Cells were detached, lysed in distilled water and serial dilutions plated to determine numbers of adherent and internalized bacteria. B BEAS-2B cells were stimulated with Golgicide A or DMSO for 4 h. Afterwards, cells were infected with Spn D39, MOI 1 for 9 h and bacterial replication determined. C-F Cells were treated with Golgicide A or DMSO of 4 h. Medium was exchanged and cells were stimulated with LTA or PBS for 2 h and the supernatant collected. For infection experiments, cells were infected with Spn, MOI 1 for 16 h in infection medium consisting of 1:1 fresh cell culture medium and supernatant from 1). C Experimental setup. D BEAS-2B were infected with Spn D39, MOI 1 for 9 h in a 1:1 mixture of fresh cell culture medium and conditioned medium as indicated. Additionally, bacteria were cultivated in a host cell free mixture of fresh/conditioned medium for 9 h. Resulting CFU were determined. E, F, G Concentrations of IL-6, IL-8 and IL-18 were determined in Golgicide A/LTA-conditioned cell culture supernatants. (N = 3–4; Statistics: paired two-tailed t-test (A, B, E, F, G), One-way ANOVA with Fisher’s LSD (D); * = p < 0.05; ns = not significant)