Fig. 4

Interactions between AML cells and different BM cell subpopulations investigated using the 3D humanized scaffold xenograft model. A Schematic presentation of the experimental setup. B Representative images of the tumor mass formed on the scaffolds in the presence of different mesenchymal cell fractions. C Plots representing the dry tissue weights of the tumors formed on the scaffolds in the presence of different mesenchymal cell fractions. One paired Mann Whitney was used for statistical analysis. *P < 0.05 was considered statistically significant. D Plots representing the absolute number of adipocyte cells in the sections demonstrated in Fig. S7A, assessed using the image j tool. Data are presented as mean ± SEM. The unpaired t test was used for statistical analysis. *P < 0.05 was considered statistically significant. E Interactions between AML cells and different BM cell subpopulations investigated using the fully humanized 3D scaffolds. Representative images of the tumor mass formed on the scaffolds in the presence of different mesenchymal cell fractions and AML blasts derived from 4 AML patients