Fig. 2

Molecular alterations in stroma cells of AML patients and functional analysis of AHNAK2 protein. A A heat-map illustrating DNA gene variations, identified using whole exome sequencing in samples derived from 7 AML patients. The colors represent allele frequencies of each single-nucleotide polymorphism (SNP). The right bar histogram indicates frequency across patients. B, C Nuclear and cytoplasmic quantitative analysis of AHNAK2 expression levels in MSCs and induced adipocytes, derived from 4 AML patients carrying an AHNAK2 mutation and 4 HDs, using IMARIS software. D Flow cytometry analysis of AHNAK2 expression levels in MSCs derived from 6 AML patients and 3 HDs, as well as in induced adipocytes derived from 3 AML patients and 3 HDs and in induced osteocytes derived from 7 AML patients and 4 HDs. E Real-time PCR analysis of: MSCs derived from 4 AML patients and 4 HDs; whole stroma derived from 12 AML patients and 6 HDs; induced adipocytes derived from 6 AML patients and 5 HDs. AHNAK2 was calibrated to β2M housekeeping gene. Data are presented as mean ± SEM