Fig. 6

Effects of endothelial ABL2 on LPS-induced p38MAPK activation and endothelial glycocalyx (EG) degradation in vivo. A Highly enrichment KEGG pathways for target genes of co-expressed miRNAs in bMSC-EVs. red label: terms of high targeting ratio, p < 0.001. B Western blot images and qRT-PCR analysis of ABL2 expression compared with that of Actin and gapdh in human umbilical vein endothelial cells (HUVECs) transfected with ABL2-sh sequence using lentivirus, (n = 3). NC: negative control, C Representative Western blot images and quantitative analysis of ABL-2, syndecan-1, MMP-9, pp38/p38, IL-6, and IL-1β levels compared to that of Actin in the normal (NC) and ABL2 knockdown HUVECs (ABL2-sh) (n = 3). D Immunofluorescence images of p38 (red) expression in the NC and ABL2-sh HUVECs treated as described in (B) using confocal microscopy. White arrows: p38 in the nucleus; blue: DAPI. Scale bar: 50 μm (n = 3). E Transendothelial resistance value to baseline (0 h) in HUVECs treated as described in (B), (n = 3). For C, D, and E, results were obtained at 6 h after LPS administration. Values are presented as mean ± sems; statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001