Fig. 1
bMSCs alleviate LPS-induced endothelial glycocalyx (EG) degradation and leakage in vivo. A Transmission electron microscopy images of EG in mice right ear tissues. Black arrows: the EG layer. Scale bar: 1 μm (n = 6). B Immunofluorescence images of syndecan-1 (green) and CD31 (red) in vascular endothelium (white arrows) contrast with hematoxylin and eosin staining of the upper or lower adjacent sections in mice right ear tissues; blue: DAPI. Scale bar: 50 μm (n = 6). C Western blot images and quantitative analysis of the syndecan-1 levels in mice right ear tissues compared to that of Actin (n = 6). D Ear leakage images displayed with Evans blue (EB) and quantitative analysis of ear EB leakage ratios (n = 8). E Quantitative analysis of ear swelling and thickening ratio (n = 8). EB leakage ratio = (aEBr − aEBl)/tl × 100%; aEB: EB absorbance; Swelling ratio = (wr − wl)/wl × 100%; w: weight. Thickening ratio = (tr − tl)/tl × 100%; t: thickness. r: right ear; and l: left ear. All results were obtained at 6 h after LPS administration. Values are presented as mean ± sems. Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001