Table 2 All primer sequences used in the present study
Names | Primer sequences (5’-3’) |
|---|---|
WTAP | Forward, CTTCCCAAGAAGGTTCGATTGA |
Reverse, TCAGACTCTCTTAGGCCAGTTAC | |
WTAP (mouse) | Forward, CTTCCGCGGACTGTCTCC |
Reverse, TCGTTGGTCATCTTGCACCC | |
YTHDF2 | Forward, AGCCCCACTTCCTACCAGATG |
Reverse, TGAGAACTGTTATTTCCCCATGC | |
IGF2BP3 | Forward, TCGAGGCGCTTTCAGGTAAA |
Reverse, TATCCAGCACCTCCCACTGTA | |
IGF2BP1 | Forward, AAGGGGGCCATCGAGAATTG |
Reverse, CAGGGATCAGGTGAGACTGC | |
IGF2BP2 | Forward, GGAACAAGTCAACACAGACACA |
Reverse, AACTGATGCCCGCTTAGCTT | |
ALKBH5 | Forward, ACTGAGCACAGTCACGCTTCC |
Reverse, GCCGTCATCAACGACTACCAG | |
FTO | Forward, GAAGCACTGTGGAAGAAGATGGA |
Reverse, GGCAAGGATGGCAGTCAAGAT | |
RBM15 | Forward, ATGCCTTCCCACCTTGTGAG |
Reverse, GGTCAGCGCCAAGTTTTCTC | |
YTHDF3 | Forward, TGTTGTGGACTATAATGCGTATGC |
Reverse, AAGCGAATATGCCGTAATTGGTTA | |
YTHDF1 | Forward, CAGCACCGATCCCGACATAG |
Reverse, CTGGCTTCCTGAAGACGATGA | |
TIMP4 | Forward, GTGAAGATCGGACACTACGTG |
Reverse, CTGGAAGGTGGACAGCGAGG | |
pri-miR-92b | Forward, AGAGCCAGACACAGAAGA |
Reverse, GGACACGACCGTCCACCA | |
pre-miR-92b | Forward, AGGGAAAGGCGGAAGAGA |
Reverse, ATTGCCCTACCCGCCAAGAAATGTAAGGTAT | |
miR-92b-5p | Forward, ACACTCCAGCTGGG TTTAGTGTGATAATGGC |
Reverse, CAGTGCGTGTCGTGGAGT | |
GAPDH | Forward, CGGCAAGTTCAACGGCACAGT |
Reverse, ACGCCAGTAGACTCCACGACAT | |
GAPDH (mouse) | Forward, CCAGCTACTCGCGGCTTTAC |
Reverse, AATCCGTTCACACCGACCTT | |
U6 | Forward, CTCGCTTCGGCAGCACA |
Reverse, TGGTGTCGTGGAGTCG | |
Primers used for MeRIP-qPCR | |
Pri-miR-92b | Forward, CTTCTGGGACTCCGCAAACT |
Reverse, TTGGAGGCCAGAGAGACTTG | |
TIMP4 | Forward, AACAGCCAGAAGCAGTATC |
Reverse, TGTGTAGCAGGTGGTGAT | |
Primiers used for in vitro transcription | |
Pri-miR-92b | Forward, CCGCTCGAGTAATACGACTCACTATAGGGAGAAGTCTGAGTACTT AAAGAGCAAGCGC |
Reverse, CTAGTCTAGATAGAAGAGAAAGCCTGGGAGGGT | |
Pri-miR-1–1 | Forward, CCGCTCGAGTAATACGACTCACTATAGGGAGAAAGGCTGTCCTGC TCACACA |
Reverse, CTAGTCTAGATCCCGGCCTGAGATACATAC | |