Fig. 8

WTAP knockdown inhibited LPS-induced OA chondrocyte damage by upregulating TIMP4. Chondrocyte was infected with WTAP knockdown adenovirus alone or in combination with TIMP4 knockdown adenovirus, and then treated with 40 ng/mL LPS for 48 h. A CCK-8 was detected the viability of chondrocyte. B EdU staining was applied to detect the proliferation of chondrocyte. C and E The apoptosis of chondrocyte was assessed by flow cytometry. D The caspase-3 activity was measured by the caspase-3 activity kit. F Western blotting was applied to assess the protein expression levels of MMP13, ADAMTS5, Aggrecan, and Collagen II. LPS, lipopolysaccharide; shTIMP4, TIMP4 knockdown adenovirus; shWTAP, WTAP knockdown adenovirus. N = 3. ^*P < 0.05, ^**P < 0.01, and.^***P < 0.001