Fig. 7

WTAP knockdown inhibited LPS-induced OA chondrocyte damage by regulating miR-92b-5p. Chondrocyte was infected with WTAP knockdown adenovirus alone or in combination with miR-92b-5p mimics, and then treated with 40 ng/mL LPS for 48 h. A The expression of miR-92b-5p was assessed by qRT-PCR. B The mRNA and protein expression levels of TIMP4 were measured by both qRT-PCR and western blotting. C The caspase-3 activity was evaluated by the caspase-3 activity kit. D The apoptosis of chondrocyte was evaluated by flow cytometry. E CCK-8 assay was used to detect the viability of chondrocyte. F EdU staining was used to assess the proliferation of chondrocyte. G Western blotting was applied to measure the protein expression levels of MMP13, ADAMTS5, Aggrecan, and Collagen II. LPS, lipopolysaccharide; miR mim., miR-92b-5p mimics; shWTAP, WTAP knockdown adenovirus. N = 3. ^*P < 0.05, ^**P < 0.01, and.^***P < 0.001