Fig. 4

WTAP knockdown ameliorated the OA progression in mice. A The protein expression of WTAP was evaluated by western blotting in the knee cartilage of OA mice with or without AAV-shWTAP treatment and sham mice. B The section of the knee joints was stained with H&E and Safranin O & Fast Green, respectively. C OARSI scores of the knee joints in OA mice with or without AAV-shWTAP treatment. D The protein expression levels of MMP13, ADAMTS5, Aggrecan, and Collagen II were evaluated by western blotting in the knee cartilage of OA mice with or without AAV-shWTAP treatment. OA, osteoarthritis; Sham, mice without destabilizing the medial meniscus (DMM); model, DMM surgery-induced OA mice; AAV- shWTAP, WTAP knockdown adeno-associated virus; AAV-shNC, negative control corresponding to AAV-shWTAP. N = 3 ~ 6. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001