Fig. 3

WTAP overexpression inhibited proliferation and ECM synthesis as well as promoted ECM degradation and cell apoptosis in LPS-induced OA chondrocyte injury. Chondrocyte was infected with WTAP or mock, and then treated with 40 ng/mL LPS for 24 h or 48 h. A The qRT-PCR and western blotting were applied to confirm the overexpression of WTAP in LPS-induced OA chondrocyte. B CCK-8 was applied to evaluate the viability of chondrocyte. C EdU staining was applied to detect the proliferation of chondrocyte. D and F The apoptosis of chondrocyte was evaluated by flow cytometry with Annexin V-FITC Apoptosis Detection Kit. E The caspase-3 activity was evaluated by caspase-3 activity kit. G Western blotting was applied to assess the protein expression levels of MMP13, ADAMTS5, Aggrecan, and Collagen II in chondrocyte. OA, osteoarthritis; LPS, lipopolysaccharide; WTAP, WTAP overexpression adenovirus; mock, negative control corresponding to WTAP. N = 3. ^*P < 0.05, ^**P < 0.01, and.^***P < 0.001