Table 1 Strains and plasmids used in this study
Name | Genotype or description | Source |
|---|---|---|
Strain | ||
SN148 | Mata/α arg4/arg4 leu2/leu2 his1/his1 ura3::imm434/ura3::imm434 | [20] |
HHCA1 | SN148 RPS1/rps1::CIp10 | [18] |
HHCA184 | SN148 PHR1/PHR1 crz1/crz1 | [18] |
HHCA983 | SN148 PHR1/PHR1-HA::URA3 crz1/crz1 | This study |
HHCA987 | SN148 PHR1/PHR1-HA::URA3 | This study |
HHCA1033 | SN148 PHR1/phr1::ARG4 | This study |
HHCA1064 | SN148 phr1::HIS1/phr1::ARG4 | This study |
HHCA1091 | SN148 phr1:: ARG4/phr1:: HIS1 RPS1/rps1::CIp10 | This study |
HHCA1094 | SN148 phr1:: ARG4/phr1:: HIS1 RPS1/rps1::CIp10-PHR1 | This study |
HHCA1124 | SN148 PHR1/phr1::ARG4 RPS1/rps1::CIp10 | This study |
Plasmid | ||
CIp10 | The integration vector (AmpR URA3) | [21] |
CIp10-PHR1 | The full-length PHR1 gene in CIp10 (AmpR URA3) | This study |
pFA-HA-URA3 | The HA-tag plasmid for HA integration (AmpR URA3) | [22] |