Fig. 9

Embryonic signals override the fast regulatory action of miRNA in governing the EV biogenesis pathway in luminal epithelial cells. A Luminal epithelial cells were exposed to embryonic signals, E₂ and PGE₂ (24 h), followed by miR-125b-5p delivery (12 h) to test VPS36 target gene expression (unpaired T-test). Gene expression was normalized to HPRT1 (AU), identified as the best reference gene by the NormFinder algorithm. Signal intensities (upper panels) and pixel correlations (lower panels) for CD63 and VPS36 at cell borders (B) and in whole cells (C) for miRNA delivered after embryonic signals (one-way ANOVA followed by Kruskal–Wallis post hock test, Pearson correlation coefficient). D Luminal epithelial cells were transfected with miR-125b-5p (12 h), which was followed by exposure to embryonic signals, E₂ and PGE₂ (24 h), to test VPS36 target gene expression (unpaired T-test). Gene expression was normalized to HPRT1 (AU), identified as the best reference gene by the NormFinder algorithm. Signal intensities (upper panels) and pixel correlations (lower panels) for CD63 and VPS36 at cell borders (E) and in whole cells (F) for embryonic signals delivered after miRNA (one-way ANOVA followed by Kruskal–Wallis post hock test; Pearson correlation coefficient)