Fig. 4

The spatiotemporal distribution of ALIX and RAB27A at the embryo–maternal interface changes along with the progression of pregnancy. Representative immunofluorescence images displaying the juxtaposed trophoblast–luminal epithelial interfaces and colocalization of ALIX (green) and RAB27A (red) proteins. The nucleus is depicted in blue (DAPI). Trophectoderm attaches to the uterine luminal epithelium on days 12 (A) and 16 (C) of pregnancy. Uterine glands surrounded by stromal cells on days 12 (B) and 16 (D) of pregnancy. GL – glands, LE – luminal epithelium, T – trophectoderm, ST – stromal cells