Fig. 5

EIF3H regulates ferroptosis in HCC cells. A-B Cell viability assay showing the response of LM3 (A) and Hep3B (B) cell lines to different concentrations of Erastin with or without EIF3H depletion. C-D Colony formation showing the response of LM3 (C) and Hep3B (D) cell lines to Erastin with or without EIF3H depletion. E–F Representative images illustrating LM3 (E) and Hep3B (F) cells response to Erastin (20 μM) ± Ferrostatin (1 μM) with or without EIF3H knockdown. I-K Levels of lipid ROS (I, J), GSH (K), and ferrous iron (L) in HCC cells with or without EIF3H depletion. ***P value < 0.001