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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: The deubiquitinase EIF3H promotes hepatocellular carcinoma progression by stabilizing OGT and inhibiting ferroptosis

Fig. 3

EIF3H deubiquitylates OGT. A OGT was immunoprecipitated with anti-OGT and immunoblotted with anti-HA. LM3 cells transfected with the indicated siEIF3H were treated with MG132 for 6 h before collection. B Immunoblotting to detect the ubiquitination of OGT in HEK293 cells co-transfected with Flag-OGT, HA-Ubiquitin and GFP-EIF3H (wild type or DDQ/AAA). C EIF3H removed the ubiquitin chain of OGT in a dose-dependent manner. D-E K48 or K63 Ub was co-transfected with Flag-OGT and GFP-EIF3H into HEK293 cells. After treatment with 10 μM MG132 for 6 h, cell lysates were subjected to ubiquitination assay and the ubiquitination level of OGT was detected by HA antibody

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Cell Communication and Signaling

ISSN: 1478-811X