Fig. 1

EIF3H depletion decreases O-GlcNac pathway activity and correlates with poor prognosis in hepatocellular carcinoma. A The siRNAs specific to each deubiquitinating enzyme (JAMMs family) were transfected into LM3 cells. After 48 h, cells were lysed and the O-GlcNac and OGT protein levels were analyzed by Western blot. B EIF3H depletion reduced OGT protein levels in both LM3 and HepG2 cells. C Genetic alternations of JAMMs family DUBs in HCC. The OncoPrint schematic was constructed in cBioPortal (TCGA dataset). D-E Prognostic analysis between EIF3H expression and OS (D) and RFS (E) in TCGA HCC patients. F Immunohistochemistry analysis of EIF3H and OGT expression in the Xiangya HCC cohort (n = 97), including normal liver tissue (n = 20) and HCC tumor tissues (n = 77). G Kaplan–Meier curve analysis of the EIF3H in Xiangya HCC cohort. H Correlations between EIF3H and OGT protein levels in Xiangya HCC cohort (Pearson correlation, R = 0.45). HCC, hepatocellular carcinoma; DUBs, deubiquitinating enzymes; OS, overall survival; RFS, relapse-free survival