Fig. 4
From: Effects of a novel ANLN E841K mutation associated with SRNS on podocytes and its mechanism
Kidney changes in glomerular ANLNpodKO mice. a Flow chart of the construction of ANLNpodKO mice. loxP indicates the site of gRNA insertion. F1, R1, F2, and R2 represent primer. b Sanger sequencing of the mouse ANLN gene inserted gRNA. c Representative agarose gel electrophoretogram of PCR products of mouse tail and kidney tissue DNA. M indicates Marker, + / + indicates no loxP site, flox/ + indicates that one allele is inserted with gRNA, flox/flox indicates that both alleles are inserted with gRNA, WT indicates that the fragment in the middle of the two loxP sites is not deleted, and KO indicates that the fragment in the middle of the two loxP sites is deleted. d Representative immunofluorescence images of glomerulus. Red stands for ANLN, green for WT1, and blue for DAPI. The scale bar was 10 μm. e Representative images of HE, PAS, Masson staining and TEM observation in four groups. The scale bar was 20 μm for HE and 1 μm for TEM. f The urine Albumin/Creatinine ratio of ANLNflox/flox, ANLNpodKO, ANLNflox/flox + ADR and ANLNpodKO + ADR mice. g Quantification of glomerular injury in four groups. h Quantification of slit diaphragm number on GBM in four groups. i Representative WB plots of kidney tissue in ANLNflox/flox and ANLNpod−KO mice after ADR treated. j-m WB quantification of Bax/Bcl2, C-Caspsase3/Caspsase3, CHOP in ANLNflox/flox + ADR and ANLN.podKO + ADR mice groups. n.s. indicates P > 0.05, * indicates P < 0.05, and ** indicates P < 0.01