Fig. 7
From: SUMOylation of AnxA6 facilitates EGFR-PKCα complex formation to suppress epithelial cancer growth
AnxA6 SUMOylation inhibits tumor growth of the epithelial cancer cells-xenografted nude mouse model. A-D AnxA6 knockdown promotes tumor growth in HeLa-xenografted mice. A-C 5 × 106 cells, including HeLa and HeLa-AnxA6(KD), were injected subcutaneously into male BALB/c nude mice (n = 5) individually (A). The sizes of tumors were measured at day 6, 9, 12, 15, 18 and 21 after injection (B), and the tumors were weighed (C). And the expression of pY-EGFR, EGFR, pERK1/2, ERK1/2 and AnxA6 were analyzed in HeLa and HeLa-AnxA6(KD)-xenografted tumors (D). E–G The K299 mutation of AnxA6 impaired its cancer suppression effection on A431-xenograft tumor growth. 5 × 106 cells, including A431, A431-AnxA6, and A431-AnxA6K299R, were injected subcutaneously into male BALB/c nude mice (n = 5) individually (E). The sizes of tumors were measured at day 9, 12, 15, 18, 21, 24, 27 and 30 after injection (F), and the tumors were weighed (G). H Ki-67 expression was detected by immunohistochemical staining in A431-xenograft tumors at 30 days after implantation (50 ×) (left), and the rates of staining positivity for Ki-67 were shown at right. I The K299R mutation of AnxA6 impaired its ability to inactivate the pY-EGFR and pERK1/2 in A431-xenograft tumors. The expression of pY-EGFR, EGFR, pERK1/2, ERK1/2 and AnxA6 were analyzed in A431, A431-AnxA6, and A431-AnxA6.K299R-xenografted tumors.T1-T5: tumor tissues from HeLa-xenograft nude mouse.T1-T3: tumor tissues from A431-xenograft nude mouse. *p < 0.05;**p < 0.01