Fig. 6
EtBr rescued shOPA1 or shMFN2-induced mitochondrial DNA extravasation. A Representative protein levels of cGAS, STING, p-TBK1s172, TBK1, p-IRF3s396 and IRF3 were detected by western blotting. B Quantification of A. C The mRNA levels of Dloop1, Dloop2, Dloop3, CytB, Rnr2, ND2, and ND4 in H9C2 cells were detected by qRT-PCR. D The mRNA levels of NLRP3, TNF-α, and IL-1β were detected by qRT-PCR. E Representative protein levels of LC3-II, P62, Tom20, and LAMP2 were detected by western blotting. F Quantification of E. Data are presented as mean ± SEM. *P < 0.05 vs. vehicle group, #P < 0.05 vs. shMFN2 group, &P < 0.05 vs. shOPA1 group. G Representative fluorescent images of STING. Scale bar: 25 μm