Fig. 5

HG + PA + HR aggravated cardiomyocyte injury and exacerbated cytoplasmic mitoDNA accumulation and inflammation. A Schematic diagram of H9C2 cell experiment, NS (normal condition: low glucose 5 mM), HG (high glucose: 25 mM), PA (palmitate: 800 µM). B-F The LDH activity, cells viability, MDA content, SOD-1 and HO-1 mRNA levels were detected. Data are presented as mean ± SEM. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 vs. NS group; ^#P < 0.05 and ^##P < 0.01 vs. NS + HR group; ^%P < 0.05 and ^%%P < 0.01 vs. HG + PA group. G Representative protein levels of MFN2, OPA1 and DRP1 were detected by western blotting. H Quantification of G. Data are presented as mean ± SEM. ^**P < 0.01, ^***P < 0.001. I Representative fluorescent images of 8-OHdG. Scale bar: 40 μm. J Representative protein levels of cGAS, STING, p-TBK1^s172, TBK1, p-IRF3^s396 and IRF3 were detected by western blotting. K Quantification of J. L The mRNA levels of NLRP3, TNF-α, and IL-1β were detected by qRT-PCR. Data are presented as mean ± SEM. ^***P < 0.001