Fig. 4

Overexpression of rAAV-MFN2 alleviated myocardial oxidative damage, inhibited phosph-TBK1/IRF3 pathway activity, and reduced inflammatory response in diabetic MI/R mice. A-C LDH, cTnT and CK-MB activity in serum. D MDA content in myocardial tissue. E–F mRNA level of SOD-1 and HO-1 in myocardial tissue. All data are presented as mean ± SEM, n = 6 mice per group. ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. G Representative protein levels of p-TBK1^s172, TBK1, p-IRF3^s396 and IRF3 were detected by western blotting. H Quantification of G. Data are presented as mean ± SEM. ^****P < 0.0001. I mRNA levels of NLRP3, TNF-α, and IL-1β were detected by qRT-PCR. All data are presented as mean ± SEM. ^**P < 0.01, ^****P < 0.0001. J Left ventricular ejection fraction (LVEF, n = 6). K Left ventricular fractional shortening (LVFS, n = 6). All data are presented as mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^****P < 0.0001. L Representative images of Evans blue and TTC stained heart sections after MI/R or sham operation. M Representative images of HE-stained heart sections after MI/R or sham operation