Fig. 3

Overexpression of rAAV-MFN2 regulated mitochondrial dynamics and function in diabetic MI/R mice. Three weeks before the HFD + STZ mice were stabilised;saline, rAAV-OPA1 5 × 10 ¹¹ VG/mL and rAAV-MFN2 5 × 10 ¹¹ VG/mL were injected via tail vein injection. After 3 weeks of transfection, the normal diet and HFD + STZ mice were subjected to 120 min of reperfusion after 30 min of ischaemia. A Protein levels of DRP1 were detected using western blotting. B Quantification of A. Data are presented as the mean ± SEM. ^*P < 0.05, ^**P < 0.01. C-D Representative EM images of mitochondrial morphology. Data are shown as a dot plot of the percentage of damaged mitochondria from six images in each group, ^****P < 0.0001. Scale bar: 5 µm. Zoom scale bar: 1 µm. Yellow arrow: lipid droplets. Red arrow: damaged mitochondria. Green arrow: normal mitochondria. E–H Total ATP production and ETC complex activity in cardiomyocytes. All data are presented as the mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. I The mRNA levels of Dloop1, Dloop2, Dloop3, CytB, Rnr2, ND2, and ND4 in the myocardial tissue were detected by qRT-PCR. Data are presented as the mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001