Fig. 2
Diabetic MI/R injury aggravated, mitochondrial dynamic imbalance and mitoDNA leakage, activation of cGAS-STING pathway and inflammation. The mice were subjected to 120 min of reperfusion after 30 min of ischaemia. A-C LDH, cTnT, and CK-MB activities in the serum. D MDA content in the myocardial tissue. E–F mRNA levels of SOD-1 and HO-1 in the myocardial tissue. All data are presented as mean ± SEM from six independent experiments, n = 6 mice per group. *P < 0.05. G Mitochondrial fusion and fission protein contents in myocardial tissue were detected by western blotting. H Quantification of G. I The mRNA levels of Dloop1, Dloop2, Dloop3, CytB, Rnr2, ND2, and ND4 in myocardial tissue were detected by qRT-PCR. J Representative protein levels of cGAS, STING, p-TBK1s172, TBK1, p-IRF3s396 and IRF3 detected by western blotting. K Quantification of J. L mRNA levels of NLRP3, TNF-α, and IL-1β detected by qRT-PCR. All data are presented as mean ± SEM, n = 6 mice per group. *P < 0.05 versus ND + sham group; #P < 0.05 versus ND + MI/R group; &P < 0.05 versus HFD + STZ + sham group