Fig. 7

NEK8-mediated colorectal cancer cell proliferation depended on c-MYC S405 phosphorylation. A Western blots to detect the expression level of c-MYC, cyclin D1, and CDK4 in cancer cells stably expressing Vector + c-MYC WT, NEK8 + c-MYC WT, Vector + c-MYC S405A, NEK8 + c-MYC S405A. B CCK8 assays to detect the proliferation ability of cancer cells from Fig. 7A; n = 3, P(SW48) = 0.0011 or 0.0001, P(Lovo) = 0.0025 or 0.0001. C Detecting the cellular ATP level of cancer cells from Fig. 7A; n = 3, P(SW48) < 0.0001, P(Lovo) < 0.0001. D EdU assays to investigate the DNA replication ability of cancer cells from Fig. 7A; statistical analysis shown; n = 3, P(SW48) < 0.0001, P(Lovo) < 0.0001. E The representative tumor images are shown. F The tumor growth curve is shown; n = 5, P = 0.0103, 0.0072, or 0.0035. G Statistical analysis of tumor weight; n = 5, P = 0.0121, 0.0113, or 0.0024. H Western blots to investigate the expression level of NEK8, c-MYC, and Ki-67 in tumors from Fig. 7E. I The working model is shown. All western blots were conducted three times, and similar results were found; a Student's t-test was applied for statistical analysis