Fig. 3

Deletion of NEK8 inhibited colorectal cancer cell proliferation in vivo and in vitro. A Knocking out the expression of NEK8 using the Crisp-cas9 system in SW48 and Lovo cell lines; western blots was conducted to confirm successful construction. B CCK8 assays to investigate the proliferation ability of SW48 and Lovo from Fig. 3A; n = 3, P(Left) = 0.0056 or 0.0065, P(Right) = 0.0012 or 0.0019. C, D EdU assays to investigate the DNA replication ability of SW48 and Lovo cells from Fig. 3A; representative images shown (C); statistical analysis shown (D); n = 3, P(SW48) = 0.0005, P(Lovo) = 0.0038 or 0.0011. E Detecting the cellular ATP level of SW48 and Lovo from Fig. 3A; n = 3, P(SW48) = 0.001, P(Lovo) = 0.0008 or 0.0007. F The representative tumor images are shown. G The tumor growth curve is shown; n = 5, P = 0.0048 or 0.0042. H Statistical analysis of tumor weight; n = 5, P = 0.0041 or 0.0029. All western blots were conducted three times, and similar results were found; a Student's t-test was applied for statistical analysis