Fig. 2
From: NEK8 regulates colorectal cancer progression via phosphorylating MYC
NEK8 overexpression promoted colorectal cancer cell proliferation. A Western blots confirmed the successful construction of SW48 and Lovo stably expressing Vector or NEK8. B CCK8 assays to investigate the proliferation ability of SW48 and Lovo stably expressing Vector or NEK8; n = 3, P(Left) = 0.0009, P(Right) = 0.0025. C, D EdU assays to investigate the DNA replication ability of SW48 and Lovo stably expressing Vector or NEK8; representative images shown (C); statistical analysis shown (D); n = 3, P(SW48) = 0.0047, P(Lovo) = 0.0039. E Detecting the cellular ATP level of SW48 and Lovo stably expressing Vector or NEK8; n = 3, P(SW48) = 0.0007, P(Lovo) = 0.0017. All western blots were conducted three times, and similar results were found; Student's t-test was applied for statistical analysis