Fig. 1
From: NEK8 regulates colorectal cancer progression via phosphorylating MYC
NEK8 was upregulated in colorectal cancer. A Assessing the expression level of NEK8 in colorectal cancer tissues and normal colorectal tissues using the GEPIA database. B Analysis of the expression level of NEK8 in colorectal cancer tissues and normal colorectal tissues using TCGA database; P < 0.0001. C Analysis of the expression level of NEK8 in colorectal cancer tissues and adjacent normal colorectal tissues by western blots. D Analysis of the mRNA expression level of NEK8 in colorectal cancer tissues and adjacent normal colorectal tissues by RT-PCR. E–H Analysis of the expression level of NEK8 in colorectal cancer tissues and adjacent normal colorectal tissues by IHC assays; representative images shown (E); statistical analysis of IHC scores grouped by normal and tumor (F), T stage (G), and clinical stage (H); number = 69, P (F) < 0.0001, P (G) = 0.0082, and P (H) < 0.0001. I Analysis of the overall survival time of colorectal cancer patients grouped by NEK8 expression level using the GEPIA database; P = 0.0041. J IHC assays to detect the expression level of NEK8 in colorectal cancer tissues; representative images are shown. K Kaplan–Meier Plotter of colorectal cancer patients grouped by IHC score from Fig. 1 J; P < 0.0001. All western blots were conducted three times, and similar results were found; a Student's t-test was applied for statistical analysis