Fig. 7
p-ERK, p-Drp1 and LC3II are co-expressed in OGD/R-treated SH-SY5Y cells. A Multiple immunofluorescence images of p-ERK (green), p-Drp1 (pink) and LC3II (red) proteins at 24 h after OGD/R. Nuclei were stained with Dapi (blue), scare bar: 100 μm. Detail represents the enlarged view of the rectangles in Merge. B Quantitative analysis of p-ERK + p-Drp1 + LC3II positive cell rate. All data in this figure are representative of three independent repeats and are shown as mean ±SEM. PD: PD98059 group; si-Drp1: Drp1 si-RNA sequences; Drp1S616E: Drp1S616E-GFP-M90 plasmid; *P < 0.05 vs. Control, #P < 0.05 vs. Model, &P < 0.05 vs. Drp1S616E