Fig. 5
Inhibition of ERK downregulates autophagy by reducing Drp1-dependent mitochondrial fission after OGD/R in SH-SY5Y cells. A, E, Representative immunoblot bands of LC3B, Beclin1 and p62; B-D, F-H, Quantitative analysis of LC3B, Beclin1 and p62; I. Transmission electron microscopy images of autophagosome/autolysosome (red arrows). All data in this figure are representative of three independent repeats and are shown as mean ±SEM. si-NC: Drp1 si-RNA negative control group; si-751/si-843/si1684: Drp1 si-RNA sequences; Drp1S616E: Drp1S616E-GFP-M90 plasmid; Vector: Drp1S616E negative control group. *P < 0.05 vs. Control, #P < 0.05 vs. si-NC, &P < 0.05 vs. si-843, $P < 0.05 vs. si-1684, @P < 0.05 vs. PD + si-NC