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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Inhibition of ERK downregulates autophagy via mitigating mitochondrial fragmentation to protect SH-SY5Y cells from OGD/R injury

Fig. 3

Transfection efficiency of si-RNA and overexpression plasmid. A Representative immunoblot bands of p-Drp1 and Drp1; B, C, Quantitative analysis of p-Drp1 and Drp1; D, SH-SY5Y cells transfected with Drp1S616E-GFP-M90 plasmid after 24 h observed under fluorescence microscope; E, Quantitative analysis of fluorescence intensity; F, H, Representative immunoblot bands of Mfn2 after transfected with si-Mfn2 and Mfn2 pcDNA3.1; G, I, Quantitative analysis of Mfn2. All data in this figure are representative of three independent repeats and are shown as mean ±SEM. si-NC: Drp1 si-RNA negative control group; si-751/si-843/si1684: Drp1 si-RNA sequences; Drp1S616E: Drp1S616E-GFP-M90 plasmid; Vector: Drp1S616E negative control group; si-1/si-2/si-3: Mfn2 si-RNA sequences; OE-Mfn2: Mfn2 pcDNA3.1. *P < 0.05 vs. si-NC and Control; #P < 0.05 vs. Vector

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Cell Communication and Signaling

ISSN: 1478-811X