Fig. 11

Inhibition of ERK prevents mPTP opening and protects from OGD/R injury by increment of Mfn2 in SH-SY5Y cells. A, The opening of mPTP was detected by loading with calcein AM (green fluorescence). The intensity of green fluorescence was negatively correlated with the opening of mPTP, scare bar: 200 μm. B, Quantitative analysis of calcein AM florescence intensity. C, D, LDH and CCK-8 assays were performed to detect cell injury and viability. All data in this figure are representative of three independent repeats and are shown as mean ±SEM. si-Mfn2(C): Mfn2 si-RNA without OGD/R group; PD: PD98059 group; Mfn2: Mfn2 si-RNA group. *P < 0.05 vs. Control, #P < 0.05 vs. Model, %P < 0.05 vs. PD + Mfn2