Fig. 2

ARG1 knockdown decreases collagen 1A1, fibronectin, and vimentin expression and cell migration. A RT–qPCR analysis of ARG1 gene knockdown efficiency. The mRNA expression levels of ZO-1, collagen 1A1, fibronectin, and vimentin in HLECs treated with or without TGF-β2 after ARG1 knockdown were detected by RT–qPCR (N = three). B, C The protein expression levels and quantification of ARG1, fibronectin, ZO-1, collagen 1A1, and vimentin in HLECs treated with or without TGF-β2 after ARG1 knockdown were detected by western blot analysis (N = three). D, E Representative images and quantification of HLECs on the inferior surface of the upper chamber following a Transwell assay after ARG1 knockdown (scale bar: 100 μm). F, G Representative images and quantification of the migration of HLECs transfected with siARG1 (scale bar: 400 μm). The statistical results only showed the difference between siNC + TGF-β2 group and siARG1 + TGF-β2 group. Error bars represent the mean ± SD, and comparisons were performed using one-way ANOVA. *P < 0.05, **P < 0.01, and ***P < 0.001