Fig. 5
From: Enhancer RNAs: mechanisms in transcriptional regulation and functions in diseases
The regulation factors of eRNAs production, degradation and modification. A At the initiation of transcription, the cap-binding complex (CBC) form a 7-methylguanosine (m7G) cap at the 5′-end of eRNAs; P-TEFb and BRD4 control the transcript elongation of eRNAs, which are recruited by the acetylated histone tail on the enhancer; The termination of eRNA transcription is mediated by the Integrator complex and WDR82. B The degradation of eRNAs is regulated by RNA exosome complexes. C Chemical modifications including m5C and m6A of eRNAs can stabilize eRNAs. eRNAs m5C modifications is catalyzed by NSUN7; eRNAs m6A methylation and demethylation are catalyzed by MTC and ALKBH5, respectively. MTC: METTL3/METTL14/WTAP m6A methyltransferase complex