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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: N-glycosylation acts as a switch for FGFR1 trafficking between the plasma membrane and nuclear envelope

Fig. 3

N-glycosylation shapes the interactome of FGFR1. A. Western blotting analysis of SBP-FGFR1 and SBP-FGFR1.GF affinity purification for mass spectrometry experiments. B. Quantitative MS-assessed selected proteins differentially interacting with SBP-FGFR1.GF and SBP-FGFR1 (full list available in Table S1). Nuclear-localized proteins are marked in blue, ER/Golgi proteins are marked in brown. Proteins for whose SBP-FGFR1.GF/SBP-FGFR1 ratio was around 1 or proteins with a q value above 0.05 are listed in Table S1 only. C. PLA confirmation of the interaction between FGFR1.GF and selected ER/nuclear proteins identified in MS experiments. Scale bar represent 10 µm

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