Table 1 The information of antibodies
Name of antibody | Manufacturer and catalog # | Molecular weight | Dilution used |
|---|---|---|---|
CGB | Proteintech (11615–1-AP) | 18 kDa | 3000x |
Phospho-SMAD1/5/8 | Cell Signaling Technology (13820) | 60Â kDa | 1000x |
Phospho-SMAD2 | Cell Signaling Technology (3108) | 60Â kDa | 1000x |
Phospho-SMAD3 | Cell Signaling Technology (9520) | 52Â kDa | 1000x |
SMAD1 | Cell Signaling Technology (6944) | 60Â kDa | 1000x |
SMAD2 | Cell Signaling Technology (3103) | 60Â kDa | 1000x |
SMAD3 | Cell Signaling Technology (9523) | 52Â kDa | 1000x |
SMAD4 | Cell Signaling Technology (38454) | 70Â kDa | 1000x |
α-Tubulin | Santa Cruz (sc-23948) | 55 kDa | 5000x |