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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Hepatocellular carcinoma cells remodel the pro-metastatic tumour microenvironment through recruitment and activation of fibroblasts via paracrine Egfl7 signaling

Fig. 6

Egfl7 promotes CAF recruitment and activation through ανβ3 integrin signaling. A qRT-PCR and western blotting showed integrin ανβ3, EGFR and NOTCH expression levels in liver fibroblasts (LFs). B qRT-PCR and western blotting showed integrin ανβ3, EGFR and NOTCH expression levels in CAFs and LFs stimulated and activated by Egfl7. C Transwell migration assays were used to detect the migratory ability of liver fibroblasts (LFs) cocultured with HCCLM3shEgfl7, SMMC-7721Egfl7, or control cells or recombinant Egfl7 protein (rEgfl7) after adding or not adding ανβ3 integrin neutralizing antibody. D Immunofluorescence staining detected α-SMA expression in LFs cocultured with HCCLM3shEgfl7, SMMC-7721Egfl7, or control cells or recombinant Egfl7 protein (rEgfl7) after adding or not adding ανβ3 integrin-neutralizing antibody. E Western blotting was used to analyse the activation of FAK, ERK and AKT signaling in LFs cocultured with HCCLM3shEgfl7, SMMC-7721Egfl7, or control cells or recombinant Egfl7 protein (rEgfl7) after adding or not adding ανβ3 integrin-neutralizing antibody. BSA, bovine serum albumin; rEgfl7, recombinant Egfl7 protein (5 μg/mL). ***P < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X