Fig. 3From: Hepatocellular carcinoma cells remodel the pro-metastatic tumour microenvironment through recruitment and activation of fibroblasts via paracrine Egfl7 signalingHighly metastatic HCC cells can recruit and activate LFs to yield CAFs. A, B Transwell migration assays (A) and wound-healing assays (B) were used to determine the role of highly metastatic HCC cell lines and less metastatic HCC cell lines in liver fibroblast (LF) migration. C, D Immunofluorescence staining (C) and western blotting analysis (D) of LFs using an α-SMA antibody after coculture with highly metastatic HCC cell lines and less metastatic HCC cell lines. E Collagen gel contraction assay confirmed LF contraction ability after treatment with conditioned medium from highly metastatic HCC cell lines and less metastatic HCC cell lines. **P < 0.01Back to article page