Fig. 1
From: Neutrophil extracellular traps promote angiogenesis in gastric cancer
NETs in the tissues and plasma of gastric cancer patients. a, b Plasma levels of the NET markers citrullinated histone H3-DNA (cit-H3-DNA) and myeloperoxidase DNA (MPO-DNA) in gastric cancer (GC) patients and healthy individuals measured via ELISA. Healthy individuals, n = 20; stage I, n = 20; II, n = 20; III, n = 20. c The levels of neutrophils and neutrophil extracellular trap (NET) markers CD66b, cit-H3, and MPO in gastric cancer tissues were measured via immunohistochemical staining, and the vascular abundance was observed via hematoxylin and eosin (HE) staining. d The expression area coverage of CD66b, cit-H3, and MPO was assessed via immunohistochemical staining. e Analysis of the spatial distribution of the NET markers MPO and cit-H3 and the vascular marker protein CD31 via multiple immunohistochemical staining. f The release of NETs by neutrophils and the adhesion of NETs to gastric cancer cells were observed using a scanning electron microscope. Image J software was used for the statistical analysis. All values are presented as means ± standard deviations. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001